SRX20101982: CNV-seq homo sapien: abortive samples
1 ILLUMINA (NextSeq 500) run: 5.1M spots, 183M bases, 65.9Mb downloads
Design: Sequencing Library preparation was carried out in accordance with the manufacturer's instructions. In brief, total genomic DNA was extracted from tissue samples using the Amp Genomic DNA Kit (TIANGEN Biotech, Beijing, China). After shearing the genomic DNA to an average size of 200 bp, 2.5 ng of the fragmented DNA was used to create the sequencing library. End-repair and A-tailing was then perform, followed by the ligation of 650-bp barcoded sequencing adaptors and treated DNA fragments. PCR was performed subsequently and the its product was purified for CNV-seq.
Submitted by: Fujian Provincial Maternity and Child Health Hospital
Study:
Experience of copy number variation sequencing applied in spontaneous abortionshow Abstracthide AbstractPurpose: We evaluated the value of copy number variation sequencing (CNV-seq) for analyzing chromosomal abnormalities (CA) in spontaneous abortion specimens.
Library:
Name: 20SC10593
Instrument: NextSeq 500
Strategy: OTHER
Source: GENOMIC
Selection: RANDOM PCR
Layout: SINGLE
Runs:
1 run, 5.1M spots, 183M bases, 65.9Mb